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        雌酮(E1)酶聯免疫吸附檢測試劑盒
        ELK7837
        規格: 價格:
        48 T ¥2240.00
        96 T ¥3200.00

        Overview 文獻

        Product name: E1(Estrone) ELISA Kit
        Reactivity: General
        Alternative Names: Oestrone
        Assay Type: Competitive Inhibition
        Sensitivity: 8.86 pg/mL
        Standard: 2000 pg/mL
        Detection Range: 31.25-2000 pg/mL
        Sample Type: Serum, plasma and other biological fluids
        Assay Length: 2h
        Research Area: Metabolic pathway;Endocrinology;Reproductive science;Genetic science;Hormone metabolism;
        Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with E1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to E1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E1 in the samples is then determined by comparing the OD of the samples to the standard curve.

        標準曲線

        Concentration (pg/mL) OD Corrected OD
        2000.00 0.189
        1000.00 0.367
        500.00 0.621
        250.00 0.833
        125.00 1.197
        62.50 1.522
        31.25 1.775
        0.00 2.123 0.000

        精密度

        Intra-assay Precision (Precision within an assay):CV%<8%

        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

        Inter-assay Precision (Precision between assays):CV%<10%

        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

        回收率

        Matrices listed below were spiked with certain level of recombinant E1 and the recovery rates were calculated by comparing the measured value to the expected amount of E1 in samples.
        Matrix Recovery range Average
        serum(n=5) 85-99% 92%
        EDTA plasma(n=5) 93-107% 100%
        Heparin plasma(n=5) 87-99% 97%

        線性

        The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
        Matrix 1:2 1:4 1:8 1:16
        serum(n=5) 89-98% 83-103% 89-102% 92-101%
        EDTA plasma(n=5) 79-93% 89-97% 85-94% 87-101%
        Heparin plasma(n=5) 91-99% 87-96% 96-105% 95-103%
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