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        小鼠腫瘤壞死因子α(TNFa)微量上樣酶聯免疫吸附檢測試劑盒
        ELK1387MS
        規格: 價格:
        48 T ¥1960.00
        96 T ¥2800.00

        Overview 文獻

        Product name: Mouse TNFa(Tumor Necrosis Factor Alpha) Microsample ELISA Kit
        Reactivity: Mouse
        Alternative Names: TNF Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2
        Assay Type: Sandwich
        Sensitivity: 6.1 pg/mL
        Standard: 1000 pg/mL
        Detection Range: 15.63-1000 pg/mL
        Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
        Assay Length: 3.5h
        Research Area: Cytokine;Tumor immunity;Infection immunity;
        Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TNFa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TNFa in the samples is then determined by comparing the OD of the samples to the standard curve.

        標準曲線

        Concentration (pg/mL) OD Corrected OD
        1000.00 2.054 1.971
        500.00 1.532 1.449
        250.00 1.106 1.023
        125.00 0.913 0.830
        62.50 0.453 0.370
        31.25 0.357 0.274
        15.63 0.252 0.169
        0.00 0.083 0.000

        精密度

        Intra-assay Precision (Precision within an assay):CV%<8%

        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

        Inter-assay Precision (Precision between assays):CV%<10%

        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

        回收率

        Matrices listed below were spiked with certain level of recombinant TNFa and the recovery rates were calculated by comparing the measured value to the expected amount of TNFa in samples.
        Matrix Recovery range Average
        serum(n=5) 95-107% 101%
        EDTA plasma(n=5) 87-99% 93%
        Heparin plasma(n=5) 84-98% 91%

        線性

        The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFa and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
        Matrix 1:2 1:4 1:8 1:16
        serum(n=5) 91-103% 87-98% 92-104% 82-96%
        EDTA plasma(n=5) 83-96% 79-93% 81-93% 86-98%
        Heparin plasma(n=5) 78-92% 83-96% 95-107% 79-86%
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