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        小鼠肌酸激酶MB同工酶(CKMB)酶聯免疫吸附檢測試劑盒
        ELK1286
        規格: 價格:
        48 T ¥1680.00
        96 T ¥2400.00

        Overview 文獻

        Product name: Mouse CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit
        Reactivity: Mouse
        Alternative Names: CK2; CK-MB; Creatine Kinase,Muscle/Brain
        Assay Type: Sandwich
        Sensitivity: 0.29 ng/mL
        Standard: 50 ng/mL
        Detection Range: 0.79-50 ng/mL
        Sample Type: serum, plasma, tissue homogenates and other biological fluids
        Assay Length: 3.5h
        Research Area: Enzyme & Kinase;Cardiovascular biology;
        Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CKMB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CKMB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CKMB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CKMB in the samples is then determined by comparing the OD of the samples to the standard curve.

        標準曲線

        Concentration (ng/mL) OD Corrected OD
        50.00 1.916 1.829
        25.00 1.394 1.307
        12.50 0.993 0.906
        6.25 0.796 0.709
        3.13 0.473 0.386
        1.57 0.327 0.240
        0.79 0.214 0.127
        0.00 0.087 0.000

        精密度

        Intra-assay Precision (Precision within an assay):CV%<8%

        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

        Inter-assay Precision (Precision between assays):CV%<10%

        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

        回收率

        Matrices listed below were spiked with certain level of recombinant CKMB and the recovery rates were calculated by comparing the measured value to the expected amount of CKMB in samples.
        Matrix Recovery range Average
        serum(n=5) 84-97% 90%
        EDTA plasma(n=5) 82-94% 88%
        Heparin plasma(n=5) 81-97% 89%

        線性

        The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CKMB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
        Matrix 1:2 1:4 1:8 1:16
        serum(n=5) 98-102% 86-92% 81-93% 89-98%
        EDTA plasma(n=5) 85-104% 97-106% 86-97% 92-99%
        Heparin plasma(n=5) 85-93% 87-96% 79-91% 95-103%
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