綿羊催乳素(PRL)酶聯免疫吸附檢測試劑盒-科鹿（武漢）生物科技有限責任公司

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綿羊催乳素(PRL)酶聯免疫吸附檢測試劑盒

ELK8303

規格：	價格：
48 T	￥2660.00
96 T	￥3800.00

下載說明 ①

Overview 文獻

Product name:	Sheep PRL(Prolactin) ELISA Kit
Reactivity:	Sheep
Alternative Names:	LTH; Luteotropic Hormone
Assay Type:	Sandwich
Sensitivity:	50 pg/mL
Standard:	8000 pg/mL
Range:	125-8000 pg/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Endocrinology;Reproductive science;Genetic science;Hormone metabolism;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolactin(PRL). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Prolactin(PRL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prolactin(PRL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prolactin(PRL) in the samples is then determined by comparing the OD of the samples to the standard curve.

Molecular Biology Reports

Association of birth type and LHX4 gene polymorphism with reproductive hormones, growth hormone, and prolactin in Awassi ewes

日期：2023-02-14 IF：2.742

標準曲線

Concentration (pg/mL)	OD	Corrected OD
8000.00	2.179	2.093
4000.00	1.628	1.542
2000.00	1.136	1.050
1000.00	0.975	0.889
500.00	0.532	0.446
250.00	0.273	0.187
125.00	0.256	0.170
0.00	0.086	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant PRL and the recovery rates were calculated by comparing the measured value to the expected amount of PRL in samples.

Matrix	Recovery range	Average
serum(n=5)	84-92%	87%
EDTA plasma(n=5)	95-104%	98%
Heparin plasma(n=5)	82-90%	86%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PRL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.